Journal: bioRxiv

Article Title: Selective Immune Silencing by Targeted TGF-β Agonists

doi: 10.64898/2026.01.19.700410

Figure Lengend Snippet: Design and characterization of mouse T cell-targeted TGF- β agonists. a. Schematic of the plasmid construct encoding the MSA–TGM1-targeting-arm fusion protein. b. SDS–PAGE analysis showing the molecular weight and purity of the indicated recombinant proteins. c. SDS–PAGE analysis showing the molecular weight and purity of the indicated recombinant proteins. d. Representative flow cytometry plots showing mouse CD4 and CD8α expression in mouse CD4- or CD8α-transduced HEK293 cells. e. Dose-dependent curves showing GITR, IRF4, and T-bet expression levels in in vitro –cultured CD4 T cells treated with the indicated proteins on day 3. f. Dose-dependent curves showing GITR, IRF4, and T-bet expression levels in in vitro –cultured CD8 T cells treated with the indicated proteins on day 3. g. Dose-dependent curves showing percentages of IFN-γ– and TNF-α–producing cells among in vitro –cultured CD4 T cells treated with the indicated proteins on day 3. h. Dose-dependent curves showing percentages of IFN-γ– and TNF-α–producing cells among in vitro –cultured CD8 T cells treated with the indicated proteins on day 3. i. Dose-dependent curves showing Foxp3 cell percentages in in vitro -cultured CD4 T cells, and Granzyme B–producing cell percentages in in vitro -cultured CD8 T cells treated with the indicated proteins on day 3. Data are presented as mean ± s.e.m.

Article Snippet: The following antibodies were purchased from Miltenyi Biotec: human IgA (130-113-480) and IRF4 (130-100-909).

Techniques: Plasmid Preparation, Construct, SDS Page, Molecular Weight, Recombinant, Flow Cytometry, Expressing, In Vitro, Cell Culture

Journal: bioRxiv

Article Title: Selective Immune Silencing by Targeted TGF-β Agonists

doi: 10.64898/2026.01.19.700410

Figure Lengend Snippet: a. Frequencies of immune cell subsets. b. SCENIC prediction of IRF4 and Blimp1 transcription factor activity. c. Immunoglobulin gene expression. d. UMAP plots showing cell cycle gene signature scores. e. Quantification of cell cycle gene signature scores. f. Scaled signature scores for TGF-β–inducible genes, senescence genes, and apoptosis-related genes, aggregated by sample.

Article Snippet: The following antibodies were purchased from Miltenyi Biotec: human IgA (130-113-480) and IRF4 (130-100-909).

Techniques: Activity Assay, Gene Expression

Journal: Experimental eye research

Article Title: Species variation and spatial differences in mucin expression from corneal epithelial cells

doi: 10.1016/j.exer.2016.09.001

Figure Lengend Snippet: PCR primer sequences and NCBI accession numbers for mucin genes/transcripts. The letter P denotes sequences that are predicted.

Article Snippet: Human MUC16 , Hs01065189_m1 , , {"type":"entrez-nucleotide","attrs":{"text":"NM_024690","term_id":"83367076","term_text":"NM_024690"}} NM_024690.

Techniques:

Journal: Experimental eye research

Article Title: Species variation and spatial differences in mucin expression from corneal epithelial cells

doi: 10.1016/j.exer.2016.09.001

Figure Lengend Snippet: The peripheral cornea expresses MUC1, MUC4 and MUC16 at higher levels than the central cornea. Epithelium was debrided from both the peripheral (~75% corneal surface area) and central (~25% corneal surface area) cornea and quantitative PCR was performed. In all species examined (A-rhesus macaque, B-canine, C-rabbit), mucin expression tended to be higher in the peripheral cornea when compared with the central cornea. Data represents relative mucin expression normalized to the respective housekeeping gene per species (Table 1, n = 6 eyes per species from different individuals). Error bars represent standard error of the mean. Asterisk indicates p ≤ 0.05.

Article Snippet: Human MUC16 , Hs01065189_m1 , , {"type":"entrez-nucleotide","attrs":{"text":"NM_024690","term_id":"83367076","term_text":"NM_024690"}} NM_024690.

Techniques: Real-time Polymerase Chain Reaction, Expressing

Journal: Experimental eye research

Article Title: Species variation and spatial differences in mucin expression from corneal epithelial cells

doi: 10.1016/j.exer.2016.09.001

Figure Lengend Snippet: Corneal mucin mRNA transcript expression pattern differs between species. Quantitative PCR of mucin mRNA transcript expression in humans (A), rhesus macaques (B), dogs (C) and rabbits (D). Similar patterns of mucin mRNA expression existed in humans, rhesus macaques and rabbits with MUC16 being expressed to the highest levels, followed by MUC1 and MUC4 expression being the lowest detected. The rabbit had a unique pattern of mucin mRNA expression with MUC4 being expressed at the highest level, MUC1 and MUC16 being expressed at lower levels. Data represents relative mucin expression normalized to the respective housekeeping gene per species (Table 1, n = 5 eyes per species from different individuals). Error bars represent standard error of the mean.

Article Snippet: Human MUC16 , Hs01065189_m1 , , {"type":"entrez-nucleotide","attrs":{"text":"NM_024690","term_id":"83367076","term_text":"NM_024690"}} NM_024690.

Techniques: Expressing, Real-time Polymerase Chain Reaction

Journal: Experimental eye research

Article Title: Species variation and spatial differences in mucin expression from corneal epithelial cells

doi: 10.1016/j.exer.2016.09.001

Figure Lengend Snippet: MUC16 glycoprotein was detectable in human, rhesus macaque and canine corneal epithelial extracts. Using a human-specific MUC16 antibody (OC125), large high molecular weight protein was detected a human, rhesus macaque and canine corneal epithelial extracts, whereas no protein was detected in rabbit epithelial extracts.

Article Snippet: Human MUC16 , Hs01065189_m1 , , {"type":"entrez-nucleotide","attrs":{"text":"NM_024690","term_id":"83367076","term_text":"NM_024690"}} NM_024690.

Techniques: High Molecular Weight